Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections.

Publication date: Jan 07, 2025

Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous mycobacteria, Mycobacterium abscessus, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against M. abscessus ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using HDCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on M. abscessus in a concentration- and light-dose-dependent manner. Complete inactivation of M. abscessus was achieved with 100 μM curcumin and 48 J cm light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5. 32 and 9. 53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that M. abscessus could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for M. abscessus.

Concepts Keywords
Atcc19977 Antimicrobial photodynamic therapy
Dermatology Curcumin
Lasers Curcumin
Photosensitizer Curcumin
Therapy Humans
Microscopy, Electron, Transmission
Mycobacterium abscessus
Mycobacterium abscessus
Mycobacterium Infections, Nontuberculous
Photochemotherapy
Photosensitizing Agents
Photosensitizing Agents
Reactive Oxygen Species
Reactive Oxygen Species
Skin Diseases, Bacterial

Semantics

Type Source Name
drug DRUGBANK Curcumin
disease MESH infections
disease IDO infection
disease IDO colony
disease IDO assay
disease IDO production
drug DRUGBANK Tretamine
disease MESH Mycobacterium Infections Nontuberculous
disease MESH Skin Diseases Bacterial

Original Article

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