Diagnostic value of SAT-TB in smear-negative pulmonary tuberculosis: A diagnostic accuracy study.

Publication date: Dec 13, 2024

This study aimed to evaluate the diagnostic value of rapid simultaneous RNA amplification and testing for tuberculosis (SAT-TB) in smear-negative pulmonary tuberculosis (PTB). We performed a multicenter prospective analysis of 206 patients with smear-negative suspected PTB between December 2018 and March 2022. We collected sputum or bronchoalveolar lavage fluid (BALF) for simultaneous SAT-TB and Xpert Mycobacterium tuberculosis/rifampin (MTB/RIF) assays. The efficiency of SAT-TB detection was also evaluated. The final analysis included 161 patients with smear-negative suspected PTB, of whom 114 provided sputum specimens and 47 provided BALF specimens. In sputum samples, the area under the curve, sensitivity, and specificity of SAT-TB for diagnosing PTB were 0. 75, 50. 7%, and 100. 0%, respectively, and those of the Xpert MTB/RIF assay were 0. 81, 62. 3%, and 100. 0%, respectively. The kappa coefficient k of the consistency between SAT-TB and Xpert MTB/RIF in sputum specimens was 0. 686. In BALF specimens, the area under the curve, sensitivity, and specificity of SAT-TB for diagnosing PTB were 0. 79, 57. 1%, and 100. 0%, respectively, and those of Xpert MTB/RIF were 0. 86, 76. 2%, and 96. 2%, respectively. The kappa coefficient k of the consistency between SAT-TB and Xpert MTB/RIF in BALF specimens was 0. 656. The SAT-TB and Xpert MTB/RIF assays were highly consistent in diagnosing smear-negative PTB. It is a valuable method for early detection, prevention, and managing smear-negative PTB suspects. Meanwhile, the detection efficiency and cost-effectiveness of SAT-TB are more suitable for the rapid diagnosis of smear-negative PTB in low- and middle-income countries.

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Concepts Keywords
Baltimore Adult
December Aged
Mycobacterium Bronchoalveolar Lavage Fluid
Tuberculosis Female
Humans
Male
Middle Aged
Mycobacterium tuberculosis
Prospective Studies
Sensitivity and Specificity
Sputum
Tuberculosis, Pulmonary

Semantics

Type Source Name
disease MESH pulmonary tuberculosis
disease MESH tuberculosis
pathway KEGG Tuberculosis
drug DRUGBANK Rifampicin
disease IDO assay
disease IDO nucleic acid

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