Publication date: Nov 29, 2024
Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed. We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the Mycobacterium tuberculosis MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy. We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85. 2% sensitivity and 97. 8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (p
| Concepts | Keywords |
|---|---|
| Antibodies | Diagnosis |
| Future | functionalized AuNPs |
| Mycobacterium | GeneXpert |
| Pcr | magnetic beads |
| Tuberculosis | Magneto-ELISA |
| MB-AuNP-I-PCR | |
| MPT-64 | |
| pleural TB |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | protein |
| disease | MESH | pleural tuberculosis |
| drug | DRUGBANK | Gold |
| drug | DRUGBANK | Morpholinylmercaptobenzothiazole |