Combining interferon-γ release assays and metagenomic next-generation sequencing for diagnosis of pulmonary tuberculosis: a retrospective study.

Ingentiumby Ingentium

In Tuberculosis Literature.

Publication date: Nov 18, 2024

Rapid diagnosis of pulmonary tuberculosis (PTB) is urgently needed. We aimed to improve diagnosis rates by combining tuberculosis-interferon (IFN)-γ release assays (TB-IGRA) with metagenomic next-generation sequencing (mNGS) for PTB diagnosis. A retrospective study of 29 PTB and 32 non-TB patients from our hospital was conducted between October 2022 and June 2023. Samples were processed for TB-IGRA and mNGS tests according to the manufacturer’s protocol. The levels of IFN-γ release in PTB patients were significantly higher than those in non-TB patients (604. 15 +/- 112. 18 pg/mL, and 1. 04 +/- 0. 38 pg/mL, respectively; p 

Open Access PDF

Concepts Keywords
Hospital IFN-γ
June mNGS
Retrospective Sputum smears
Tuberculosis TB-IGRA
Tuberculosis

Semantics

Type Source Name
pathway REACTOME Release
disease MESH pulmonary tuberculosis
disease MESH tuberculosis
pathway KEGG Tuberculosis
pathway REACTOME Reproduction
disease MESH Infectious Diseases
drug DRUGBANK Saquinavir
disease MESH emergency
disease MESH death
drug DRUGBANK Gold
disease MESH morbidity
disease IDO assay
disease IDO blood
disease MESH inflammation
pathway REACTOME Immune System
disease MESH mixed infections
disease IDO pathogen
disease MESH infections
disease IDO history
disease IDO infection
disease IDO nucleic acid
disease IDO protein
drug DRUGBANK Pentaerythritol tetranitrate
disease IDO quality
disease IDO bacteria
drug DRUGBANK Coenzyme M
disease MESH Hepatitis
disease IDO cell
disease MESH tic
disease MESH misdiagnoses
disease MESH missed diagnoses
disease IDO production
drug DRUGBANK Indoleacetic acid
disease MESH weight loss
disease MESH pneumonia
disease MESH bronchitis
disease MESH respiratory infections
disease MESH immunocompromised patients
disease MESH HIV infection
pathway REACTOME HIV Infection
disease MESH cancer
drug DRUGBANK BCG vaccine
disease MESH latent tuberculosis infection
disease MESH Pneumocystis Jirovecii Pneumonia
disease MESH aspergillosis
drug DRUGBANK (S)-Des-Me-Ampa
disease MESH spinal tuberculosis
drug DRUGBANK Guanosine
disease IDO susceptibility

Original Article

Leave a Comment

Your email address will not be published. Required fields are marked *